Nonantibiotic macrolides prevent human neutrophil elastase-induced mucus stasis and airway surface liquid volume depletion

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Nonantibiotic macrolides prevent human neutrophil elastase-induced mucus stasis and airway surface liquid volume depletion.

Mucus clearance is an important component of the lung's innate defense system. A failure of this system brought on by mucus dehydration is common to both cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). Mucus clearance rates are regulated by the volume of airway surface liquid (ASL) and by ciliary beat frequency (CBF). Chronic treatment with macrolide antibiotics is known ...

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Thick airway surface liquid volume and weak mucin expression in pendrin-deficient human airway epithelia

Pendrin is an anion exchanger whose mutations are known to cause hearing loss. However, recent data support the linkage between pendrin expression and airway diseases, such as asthma. To evaluate the role of pendrin in the regulation of the airway surface liquid (ASL) volume and mucin expression, we investigated the function and expression of pendrin and ion channels and anion exchangers. Human...

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Non-Antibiotic Macrolides Prevent Human

4 Robert Tarran, Juan R. Sabater, Tainya C. Clarke, 5 Chong D. Tan, Catrin M. Davies, Jia Liu, Arthur Yeung, 6 Alaina L. Garland, M. Jackson Stutts, William M. Abraham, 7 Gary Phillips, William R. Baker, Clifford D. Wright, Sibylle Wilbert 8 9 Cystic Fibrosis/Pulmonary Research and Treatment Center, University of North Carolina, 10 Chapel Hill, NC, 27599; Gilead Sciences, Inc., Seattle, WA, 981...

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Ezrin/Exocyst complex regulates mucin 5AC secretion induced by neutrophil elastase in human airway epithelial cells.

BACKGROUND/AIM Increased mucin secretion is a characteristic feature of many chronic airway diseases, particularly during periods of exacerbation; however, the exact mechanism of mucin secretion remains unclear. Ezrin, which is a specific marker of apical membranes, is predominantly concentrated in exocyst-rich cell surface structures, crosslinking the actin cytoskeleton with the plasma membran...

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Neutrophil elastase induces mucus cell metaplasia in mouse lung.

Goblet cell hyperplasia in the superficial airway epithelia is a signature pathological feature of chronic bronchitis and cystic fibrosis. In these chronic inflammatory airway diseases, neutrophil elastase (NE) is found in high concentrations in the epithelial lining fluid. NE has been reported to trigger mucin secretion and increase mucin gene expression in vitro. We hypothesized that chronic ...

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ژورنال

عنوان ژورنال: American Journal of Physiology-Lung Cellular and Molecular Physiology

سال: 2013

ISSN: 1040-0605,1522-1504

DOI: 10.1152/ajplung.00292.2012